Monoubiquitylation of Histone H2B and Gene Regulation in Saccharomyces Cerevisiae

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Previous studies have shown that covalent modifications of core histone proteins affect chromatin structure and function. This summer we specifically looked at monoubiquitylation of histone H2B in Saccharomyces cerevisiae, commonly known as baker’s yeast. Through the use of western blot techniques and analysis we detected monoubiquitylation in multiple strains of yeast. The second part of this project specifically focused on the glycolytic/gluconeogenic pathways in yeast metabolism during the switch from anaerobic fermentation to aerobic