Regulation of Genes Involved in Lipid Absorption by Bacterial Conditioned Media

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CONFERENCE: IMSAloquium Student Investigation Showcase

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Park, Grace
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Microbial dysbiosis; triggered by diet, environment, and host genetics; is associated with extra-intestinal disorders. Preliminary data showed that high fat diets significantly increased the abundance of the family Clostridiaceae in the small intestine and that diet-mediated dysbiosis drives lipid absorption in the small intestine causing increased adiposity. Therefore, it was hypothesized that Clostridium scindens would directly impact expression of fatty acid transporters or processing enzymes and enteroendrocrine hormones. To test this hypothesis, ileal enteroids were treated with conditioned media (CM) from C. scindens for 24 and 48 hours and mRNA levels of target genes including cluster of differentiation (cd)36, cholescystokinin, secretin, monoacylglyceride transferase (Mogat2), and peroxisome proliferator activated receptor gamma (pparg2) were measured. CM from Allobaculum e14 and Lactobacillus johnsonii were used as controls. C. scindens and L. johnsonii CM media increased cd36 expression at 48 hours, but not significantly. L. johnsonii CM significantly decreased mogat2 expression at 24 and 48 hours compared to control media. Other genes measured were not significantly altered by CM. These results suggest interaction between bacterial CM and small intestinal gene expression. However, the importance of these changes on lipid absorption and transport requires further experimentation.