Determination of Haloperidol Levels in Rat Serum by High-Performance LiquidChromatography: A Collaborative Study

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CONFERENCE: Posters-at-the-Capitol

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As part of a broader study designed to assess the effects of antipsychotic drugs on the behavioral consequences of hippocampal damage, a method for the determination of haloperidol in rat serum by high-performance liquid chromatography (HPLC) is described. These data are necessary to establish a correlation between the amount of drug administered to the rats (via drinking water), the actual concentration in the blood, and the behavioral effects observed in clinical studies. The method uses two liquid-liquid extractions and trazodone as an internal standard to evaluate extraction recovery. Standard curves have been generated using spiked mobile phase samples. Haloperidol and trazodone will be extracted from serum samples and compared to these standard curves.