Impact of green tea for colitis-associated colorectal cancer prevention in a multigenerational murine model incorporating the total Western diet.

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CONFERENCE: Research Week

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Phatak, Sumira
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Colorectal cancer (CRC) is the second leading cause of US cancer death, yet Americans routinely consume highly processed foods that are energy-dense and nutrient-poor, contributing to chronic inflammation. Other foods are known to contain bioactive molecules that protect against cancer by virtue of antioxidant properties. Over 27,000 research articles are published on green tea, a popular beverage, second worldwide only to water. Previous work has shown that green tea polyphenols decrease body fat composition, reverse insulin resistance, reduce cardiovascular disease risk, protect against pathogenic bacteria, improve brain function, increase lifespan, and suppress inflammatory processes that contribute to carcinogenesis. The objective of this project was to determine the efficacy of green tea extract (GTE) for CRC prevention after multigenerational exposure to standard or Western type diets. We hypothesized that GTE would reduce suppress development of inflammation-associated CRC in third-generation offspring, regardless of ancestral diet exposure. C57BL/6J mice were bred for three generations, during which they were fed a standard AIN93G diet or the total Western diet for rodents (TWD), during only the F0 generation, the F0 through F3 generations, or only the F3 generation. The AOM+DSS model of inflammation-associated CRC was employed in F3 offspring and mice were provided either plain drinking water or GTE supplementation. GTE decreased terminal body weight and relative fat mass in F3 females directly exposed to TWD compared to their counterpArts provided water. GTE consumption decreased tumor multiplicity and burden in both sexes after multi-generation exposure to TWD, as well as relative spleen mass in females (p=0.0083). GTE increased relative cecum mass (p=0.0113) in females after trans-generational TWD exposure. Preliminary analyses suggest that GTE supplementation reduced CRC severity only after cumulative TWD exposure over multiple generations.