Expression of Class I Histone Deacetylases in Insect Cells

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Bryan, Erin E
Worcester Polytechnic Institute
histone deacetylase; Histone deacetylase; Transcription factors
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"Histone deacetylases (HDACs) have become one of the leading areas of research for cancer, neurodegenerative diseases, diabetes, obesity, and inflammation. Although HDACs are currently expressible in mammalian cultures and yeast, it is important to explore other cost effective options. Here it is shown that class I HDACs are expressible in insect cells. As well as expressing full length domains for class I HDACs, predicted active domains have also been expressed. This information can be utilized in many areas for future research including identifying unique sites to allow development of specific inhibitors for each HDAC, and developing a better understanding of the specific role of each HDAC. "