Antagonistic Chromatin Remodeling Complexes, Mbd3 and Brg1, Regulate a Set of Common Target Genes in Embryonic Stem Cells

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Li, Ruowang
Worcester Polytechnic Institute
Biology and Biotechnology
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Two chromatin remodeling factors Mbd3 and Brg1 affect the pluripotency in mouse embryonic stem cells. KD of the two factors showed that they oppositely affect a common set of genes. Genome wide mapping of Mbd3 and Brg1 using ChIP-Seq showed that the two factors preferentially bind to promoter regions and physically interact in ES cells. Mbd3 binding is found to be enriched at Polycomb and bivalent target genes marked with H3K4me3 and H3K27me3.