Cloning and characterization of two putative glucuronolactonases from Arabidopsis thaliana involved in ascorbate degradation

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Belisle, Melinda
Worcester Polytechnic Institute
Biology and Biotechnology
artifact description
Vitamin C (ascorbic acid, AsA) is the most abundant water-soluble antioxidant in plants and animals. Two plant mutants were discovered which contained high foliar AsA levels compared to controls.160 We hypothesized that these two genes are involved in AsA degradation and have performed further studies to confirm their participation in AsA metabolism.160 This work focused on cloning of ORFs, making constructs for expression in bacteria and in planta, and transformation of wild type and Arabidopsis homozygous knockouts with appropriate constructs.