Analysis of adhesion protein DIG-1 in C. elegans.

Publication Year:
2005

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Repository URL:
https://digitalcommons.wpi.edu/mqp-all/4529; https://digitalcommons.wpi.edu/cgi/viewcontent.cgi?article=5528&context=mqp-all
Author(s):
Rosinha, Elizabeth Merry; Dasso, Robert Anthony
Publisher(s):
Worcester Polytechnic Institute
Tags:
Biology and Biotechnology; Biochemistry
artifact description
DIG-1 appears to be an adhesion protein involved in neuron guidance during C. elegans nervous system development. This MQP attempted to show recognition of, as well as specificity to DIG-1 by anti-con3, a previously raised antibody to the C-terminus. Various methods of cutting the protein were employed and analysis was performed using polyacrylamide gel electrophoresis. Fluorescence was also used to look at in vivo localization of DIG-1. Results suggest that anti-con3 does not specifically recognize DIG-1 from a protein homogenate.