Isolation and characterization of microsatellite markers in the lowland leopard frog (Rana yavapaiensis) and the relict leopard frog (R. onca), two declining frogs of the North American desert southwest.

Citation data:

Molecular ecology resources, ISSN: 1755-098X, Vol: 9, Issue: 1, Page: 199-202

Publication Year:
2009
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Repository URL:
https://digitalscholarship.unlv.edu/pli_fac_articles/4; http://ezproxy.library.unlv.edu/login?url=http://dx.doi.org/10.1111/j.1755-0998.2008.02343.x
PMID:
21564602
DOI:
10.1111/j.1755-0998.2008.02343.x
Author(s):
Savage, Anna E.; Jaeger, Jef R., Ph.D.
Publisher(s):
Wiley-Blackwell
Tags:
Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Anuran; Microsatellite; Polymorphism; Rana onca; Rana yavapaiensis; Animal Sciences; Biology; Environmental Sciences; Life Sciences; Molecular Biology; Zoology
article description
We characterized 15 microsatellite loci for the lowland leopard frog (Rana yavapaiensis) and the relict leopard frog (R. onca) for future studies of population genetic structure and relatedness. Analysis of 20 individuals from single populations of each species showed that all markers were polymorphic in at least one species. Observed and expected heterozygosities ranged from 0 to 0.94 and from 0.11 to 0.85, respectively, and there were three to 11 alleles per locus. No loci were in linkage disequilibrium, but six loci deviated significantly from Hardy-Weinberg equilibrium, and the presence of a null allele was detected in two of these loci.