Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen.

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BMC genomics, ISSN: 1471-2164, Vol: 10, Issue: 1, Page: 308

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Garcia-Reyero, Natàlia; Kroll, Kevin J; Liu, Li; Orlando, Edward F; Watanabe, Karen H; Sepúlveda, María S; Villeneuve, Daniel L; Perkins, Edward J; Ankley, Gerald T; Denslow, Nancy D
Springer Nature
Biochemistry, Genetics and Molecular Biology; Biotechnology; Genetics
article description
Aquatic organisms are continuously exposed to complex mixtures of chemicals, many of which can interfere with their endocrine system, resulting in impaired reproduction, development or survival, among others. In order to analyze the effects and mechanisms of action of estrogen/anti-estrogen mixtures, we exposed male fathead minnows (Pimephales promelas) for 48 hours via the water to 2, 5, 10, and 50 ng 17alpha-ethinylestradiol (EE2)/L, 100 ng ZM 189,154/L (a potent antiestrogen known to block activity of estrogen receptors) or mixtures of 5 or 50 ng EE(2)/L with 100 ng ZM 189,154/L. We analyzed gene expression changes in the gonad, as well as hormone and vitellogenin plasma levels.