Chlamydomonas flagella. II. The distribution of tubulins 1 and 2 in the outer doublet microtubules.

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The Journal of cell biology, ISSN: 0021-9525, Vol: 54, Issue: 3, Page: 540-55

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Witman, George B.; Carlson, K.; Rosenbaum, Joel L.
Rockefeller University Press
Biochemistry, Genetics and Molecular Biology; Acrylamides; Algae, Green; Chlamydomonas; Densitometry; Detergents; Electrophoresis; Flagella; Isoelectric Focusing; Microscopy, Electron; Microtubules; Models, Structural; Proteins; Cell Biology
article description
Quantitative ultrastructural analysis and quantitative gel electrophoresis of preparations of selectively solubilized Chlamydomonas outer doublets indicated that tubulins 1 and 2 were present in both the A tubule and the B tubule, and that only tubulin 1 was present in the three protofilaments which form the wall ("partition") between the lumens of the A and B tubules. The data suggested that the remaining protofilaments of the outer doublet were grouped together in pairs containing the same type of tubulin, pairs containing tubulin 1 alternating with pairs containing tubulin 2. These findings were used to construct models for the arrangement of the two tubulins in the outer doublet. Further analysis by isoelectric focusing resolved tubulins 1 and 2 into at least five bands.