Single particle analysis of relaxed and activated muscle thin filaments.

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Journal of molecular biology, ISSN: 0022-2836, Vol: 346, Issue: 3, Page: 761-72

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Pirani, Alnoor; Xu, Chen; Hatch, Victoria; Craig, Roger W.; Tobacman, Larry S.; Lehman, William
Elsevier BV
Biochemistry, Genetics and Molecular Biology; Actins; Animals; Binding Sites; Calcium; Cattle; Image Processing, Computer-Assisted; Microscopy, Electron; Models, Molecular; Multiprotein Complexes; Muscle Contraction; Muscle Proteins; Muscle Relaxation; Muscle, Skeletal; Myocardial Contraction; Myocardium; Rabbits; Tropomyosin; Troponin; Cell Biology
article description
The movement of tropomyosin from actin's outer to its inner domain plays a key role in sterically regulating muscle contraction. This movement, from a low Ca2+ to a Ca2+-induced position has been directly demonstrated by electron microscopy and helical reconstruction. Solution studies, however, suggest that tropomyosin oscillates dynamically between these positions at all Ca2+ levels, and that it is the position of this equilibrium that is controlled by Ca2+. Helical reconstruction reveals only the average position of tropomyosin on the filament, and not information on the local dynamics of tropomyosin in any one Ca2+ state. We have therefore used single particle analysis to analyze short filament segments to reveal local variations in tropomyosin behavior. Segments of Ca2+-free and Ca2+ treated thin filaments were sorted by cross-correlation to low and high Ca2+ models of the thin filament. Most segments from each data set produced reconstructions matching those previously obtained by helical reconstruction, showing low and high Ca2+ tropomyosin positions for low and high Ca2+ filaments. However, approximately 20% of segments from Ca2+-free filaments fitted best to the high Ca2+ model, yielding a corresponding high Ca2+ reconstruction. Conversely, approximately 20% of segments from Ca2+-treated filaments fitted best to the low Ca2+ model and produced a low Ca2+ reconstruction. Hence, tropomyosin position on actin is not fixed in either Ca2+ state. These findings provide direct structural evidence for the equilibration of tropomyosin position in both high and low Ca2+ states, and for the concept that Ca2+ controls the position of this equilibrium. This flexibility in the localization of tropomyosin may provide a means of sterically regulating contraction at low energy cost.