Flavivirus-cross-reactive, HLA-DR15-restricted epitope on NS3 recognized by human CD4+ CD8- cytotoxic T lymphocyte clones.

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The Journal of general virology, ISSN: 0022-1317, Vol: 76 ( Pt 9), Issue: 9, Page: 2243-9

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Kurane, Ichiro; Okamoto, Yuji; Dai, Li Chen; Zeng, Lingling; Brinton, Margo A.; Ennis, Francis A.
Microbiology Society
Immunology and Microbiology; Amino Acid Sequence; Animals; Antigens, CD8; Base Sequence; CD4-Positive T-Lymphocytes; Cercopithecus aethiops; Clone Cells; Cross Reactions; DNA; Epitope Mapping; Flavivirus; HLA-DR Antigens; Humans; Molecular Sequence Data; RNA Helicases; Receptors, Antigen, T-Cell, alpha-beta; Sequence Homology, Amino Acid; Serine Endopeptidases; T-Lymphocytes, Cytotoxic; Vero Cells; Viral Nonstructural Proteins; Life Sciences; Medicine and Health Sciences
article description
The role of flavivirus-cross-reactive T lymphocytes in recovery from and pathogenesis of flavivirus infections is not known. In the present paper, we have defined a flavivirus-cross-reactive epitope recognized by two CD4+ CD8- cytotoxic T lymphocyte (CTL) clones, JK4 and JK43. The T cell clones were established from the peripheral blood T lymphocytes of a dengue-4-immune donor, using a limiting-dilution method with dengue-4 antigen. These two T cell clones were cross-reactive for dengue virus types 1, 2, 3 and 4, yellow fever virus and West Nile virus, and recognized NS3 protein. The smallest synthetic peptide recognized by these T cell clones was an identical 9 amino acid peptide which contains amino acids 146 to 154 (VIGLYGNGV) of dengue-4 NS3. HLA-DR15 was the restriction allele for recognition of this epitope by JK4 and JK43. JK4 and JK43 both used T cell receptor V alpha 8, but JK4 used V beta 8 and JK43 used V beta 2. This result indicates that this epitope is recognized by two flavivirus-cross-reactive CD4+ T cell clones which originated from different T cells in vivo.