A construct with fluorescent indicators for conditional expression of miRNA.

Citation data:

BMC biotechnology, ISSN: 1472-6750, Vol: 8, Issue: 1, Page: 77

Publication Year:
2008
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Citations 29
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Repository URL:
http://jdc.jefferson.edu/pacbfp/45; https://escholarship.umassmed.edu/oapubs/2015
PMID:
18840295
DOI:
10.1186/1472-6750-8-77
PMCID:
PMC2569932
Author(s):
Qiu, Linghua; Wang, Hongyan; Xia, Xugang; Zhou, Hongxia; Xu, Zuoshang
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology; Thomas Jefferson University; Department of Pathology Anatomy and Cell Biology; 3' Untranslated Regions; Extracellular Matrix Proteins; Fluorescent Dyes; Gene Expression Regulation; Green Fluorescent Proteins; Integrases; Introns; Ketoglutarate Dehydrogenase Complex; Luminescent Proteins; MicroRNAs; Protein-Lysine 6-Oxidase; RNA Interference; Medical Biotechnology; Medical Cell Biology; 3' Untranslated Regions; Extracellular Matrix Proteins; Fluorescent Dyes; Gene Expression Regulation; Green Fluorescent Proteins; Integrases; Introns; Ketoglutarate Dehydrogenase Complex; Luminescent Proteins; MicroRNAs; Protein-Lysine 6-Oxidase; RNA Interference; Life Sciences; Medicine and Health Sciences
article description
Transgenic RNAi holds promise as a simple, low-cost, and fast method for reverse genetics in mammals. It may be particularly useful for producing animal models for hypomorphic gene function. Inducible RNAi that permits spatially and temporally controllable gene silencing in vivo will enhance the power of transgenic RNAi approach. Furthermore, because microRNA (miRNA) targeting specific genes can be expressed simultaneously with protein coding genes, incorporation of fluorescent marker proteins can simplify the screening and analysis of transgenic RNAi animals.