Distinct functions for the Drosophila piRNA pathway in genome maintenance and telomere protection.

Citation data:

PLoS genetics, ISSN: 1553-7404, Vol: 6, Issue: 12, Page: e1001246

Publication Year:
2010
Usage 7381
Full Text Views 7124
Downloads 153
Abstract Views 70
Views 34
Captures 121
Readers 79
Exports-Saves 42
Citations 51
Citation Indexes 51
Repository URL:
https://escholarship.umassmed.edu/oapubs/2360
PMID:
21179579
DOI:
10.1371/journal.pgen.1001246; 10.1371/journal.pgen.1001246.g001; 10.1371/journal.pgen.1001246.g002; 10.1371/journal.pgen.1001246.g003; 10.1371/journal.pgen.1001246.g004
PMCID:
PMC3003142; 3003142
Author(s):
Jaspreet S. Khurana; Jia Xu; Zhiping Weng; William E. Theurkauf; R. Scott Hawley
Publisher(s):
Public Library of Science (PLoS); Figshare
Tags:
Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Medicine; Genetics; disrupt; telomere; Animals; Argonaute Proteins; Chromosomal Proteins, Non-Histone; Drosophila Proteins; Drosophila melanogaster; *Genome, Insect; Meiosis; Peptide Initiation Factors; RNA Helicases; RNA, Small Interfering; Signal Transduction; Telomere; Biochemistry, Biophysics, and Structural Biology; Genetics and Genomics; Life Sciences; Medicine and Health Sciences; pirna; mutant; fusions; functions; pathway; genome; linked; chromosome; containing; telomeric; transposon
article media
article description
Transposons and other selfish DNA elements can be found in all phyla, and mobilization of these elements can compromise genome integrity. The piRNA (PIWI-interacting RNA) pathway silences transposons in the germline, but it is unclear if this pathway has additional functions during development. Here we show that mutations in the Drosophila piRNA pathway genes, armi, aub, ago3, and rhi, lead to extensive fragmentation of the zygotic genome during the cleavage stage of embryonic divisions. Additionally, aub and armi show defects in telomere resolution during meiosis and the cleavage divisions; and mutations in lig-IV, which disrupt non-homologous end joining, suppress these fusions. By contrast, lig-IV mutations enhance chromosome fragmentation. Chromatin immunoprecipitation studies show that aub and armi mutations disrupt telomere binding of HOAP, which is a component of the telomere protection complex, and reduce expression of a subpopulation of 19- to 22-nt telomere-specific piRNAs. Mutations in rhi and ago3, by contrast, do not block HOAP binding or production of these piRNAs. These findings uncover genetically separable functions for the Drosophila piRNA pathway. The aub, armi, rhi, and ago3 genes silence transposons and maintain chromosome integrity during cleavage-stage embryonic divisions. However, the aub and armi genes have an additional function in assembly of the telomere protection complex.