Investigating Methodological Differences in the Assessment of Dendritic Morphology of Basolateral Amygdala Principal Neurons-A Comparison of Golgi-Cox and Neurobiotin Electroporation Techniques.

Citation data:

Brain sciences, ISSN: 2076-3425, Vol: 7, Issue: 12, Page: 165

Publication Year:
2017
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Repository URL:
https://escholarship.umassmed.edu/oapubs/3324
PMID:
29257086
DOI:
10.3390/brainsci7120165
Author(s):
Klenowski, Paul M.; Wright, Sophie E.; Mu, Erica W. H.; Noakes, Peter G.; Lavidis, Nickolas A.; Bartlett, Selena E.; Bellingham, Mark C.; Fogarty, Matthew J.
Publisher(s):
MDPI AG
Tags:
Neuroscience; Golgi–Cox; basolateral amygdala; dendrites; neurobiotin; principal neuron; spines; Neuroscience and Neurobiology
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article description
Quantitative assessments of neuronal subtypes in numerous brain regions show large variations in dendritic arbor size. A critical experimental factor is the method used to visualize neurons. We chose to investigate quantitative differences in basolateral amygdala (BLA) principal neuron morphology using two of the most common visualization methods: Golgi-Cox staining and neurobiotin (NB) filling. We show in 8-week-old Wistar rats that NB-filling reveals significantly larger dendritic arbors and different spine densities, compared to Golgi-Cox-stained BLA neurons. Our results demonstrate important differences and provide methodological insights into quantitative disparities of BLA principal neuron morphology reported in the literature.