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An optimized two-finger archive for ZFN-mediated gene targeting.
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Nature methods, ISSN: 1548-7105, Vol: 9, Issue: 6, Page: 588-90
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- Biochemistry, Genetics and Molecular Biology; Zinc Fingers; Gene Targeting; Genetics and Genomics
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The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.