An optimized two-finger archive for ZFN-mediated gene targeting.

Citation data:

Nature methods, ISSN: 1548-7105, Vol: 9, Issue: 6, Page: 588-90

Publication Year:
2012
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Citations 87
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Repository URL:
https://escholarship.umassmed.edu/pgfe_pp/186
PMID:
22543349
DOI:
10.1038/nmeth.1994
PMCID:
PMC3443678
Author(s):
Gupta, Ankit; Rayla, Amy L.; Lakshmanan, Abirami; Wolfe, Scot A.; Christensen, Ryan G.; Stormo, Gary D.
Publisher(s):
Springer Nature
Tags:
Biochemistry, Genetics and Molecular Biology; Zinc Fingers; Gene Targeting; Genetics and Genomics
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article description
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.