Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle Differentiation.

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PloS one, ISSN: 1932-6203, Vol: 11, Issue: 1, Page: e0146893

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10.1371/journal.pone.0146893; 10.1371/journal.pone.0146893.g005; 10.1371/journal.pone.0146893.t003; 10.1371/journal.pone.0146893.g004; 10.1371/journal.pone.0146893.g010; 10.1371/journal.pone.0146893.g001; 10.1371/journal.pone.0146893.g008; 10.1371/journal.pone.0146893.t001; 10.1371/journal.pone.0146893.g006; 10.1371/journal.pone.0146893.t002; 10.1371/journal.pone.0146893.g007; 10.1371/journal.pone.0146893.g002; 10.1371/journal.pone.0146893.g003; 10.1371/journal.pone.0146893.g009
PMC4729438; 4729438
Eugénie Ansseau; Jocelyn O. Eidahl; Céline Lancelot; Alexandra Tassin; Christel Matteotti; Cassandre Yip; Jian Liu; Baptiste Leroy; Céline Hubeau; Cécile Gerbaux; Samuel Cloet; Armelle Wauters; Sabrina Zorbo; Pierre Meyer; Isabelle Pirson; Dalila Laoudj-Chenivesse; Ruddy Wattiez; Scott Q. Harper; Alexandra Belayew; Frédérique Coppée; Michael Kyba Show More Hide
Public Library of Science (PLoS); Figshare
Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Biophysics; Biochemistry; Cell Biology; Genetics; Molecular Biology; Physiology; Biological Sciences not elsewhere classified; Developmental Biology; Cancer; GST; DUX 4c interactions; LIM; DUX 4c overexpression; 1QBP; LMCD; DUX 4; DUX 1; cytoplasmic proteins; FUS; filament protein desmin; muscle cell cultures; Large muscle RNPs; Homologous Transcription Factors DUX 4; carboxyl terminal domain; SFPQ; DUX 4 partner; SRSF; Desmin filaments link; Muscle Differentiation Hundreds; DUX 4c Associate; Proximal Ligation Assay; FSHD; III; RBM; 69999 Biological Sciences not elsewhere classified; Molecular Genetics; Musculoskeletal Diseases; Nervous System Diseases
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Hundreds of double homeobox (DUX) genes map within 3.3-kb repeated elements dispersed in the human genome and encode DNA-binding proteins. Among these, we identified DUX4, a potent transcription factor that causes facioscapulohumeral muscular dystrophy (FSHD). In the present study, we performed yeast two-hybrid screens and protein co-purifications with HaloTag-DUX fusions or GST-DUX4 pull-down to identify protein partners of DUX4, DUX4c (which is identical to DUX4 except for the end of the carboxyl terminal domain) and DUX1 (which is limited to the double homeodomain). Unexpectedly, we identified and validated (by co-immunoprecipitation, GST pull-down, co-immunofluorescence and in situ Proximal Ligation Assay) the interaction of DUX4, DUX4c and DUX1 with type III intermediate filament protein desmin in the cytoplasm and at the nuclear periphery. Desmin filaments link adjacent sarcomere at the Z-discs, connect them to sarcolemma proteins and interact with mitochondria. These intermediate filament also contact the nuclear lamina and contribute to positioning of the nuclei. Another Z-disc protein, LMCD1 that contains a LIM domain was also validated as a DUX4 partner. The functionality of DUX4 or DUX4c interactions with cytoplasmic proteins is underscored by the cytoplasmic detection of DUX4/DUX4c upon myoblast fusion. In addition, we identified and validated (by co-immunoprecipitation, co-immunofluorescence and in situ Proximal Ligation Assay) as DUX4/4c partners several RNA-binding proteins such as C1QBP, SRSF9, RBM3, FUS/TLS and SFPQ that are involved in mRNA splicing and translation. FUS and SFPQ are nuclear proteins, however their cytoplasmic translocation was reported in neuronal cells where they associated with ribonucleoparticles (RNPs). Several other validated or identified DUX4/DUX4c partners are also contained in mRNP granules, and the co-localizations with cytoplasmic DAPI-positive spots is in keeping with such an association. Large muscle RNPs were recently shown to exit the nucleus via a novel mechanism of nuclear envelope budding. Following DUX4 or DUX4c overexpression in muscle cell cultures, we observed their association with similar nuclear buds. In conclusion, our study demonstrated unexpected interactions of DUX4/4c with cytoplasmic proteins playing major roles during muscle differentiation. Further investigations are on-going to evaluate whether these interactions play roles during muscle regeneration as previously suggested for DUX4c.