Advances in the Diagnosis of Human Opisthorchiasis: Development of Opisthorchis viverrini Antigen Detection in Urine.

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PLoS neglected tropical diseases, ISSN: 1935-2735, Vol: 9, Issue: 10, Page: e0004157

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10.1371/journal.pntd.0004157; 10.1371/journal.pntd.0004157.g003; 10.1371/journal.pntd.0004157.g002; 10.1371/journal.pntd.0004157.t001; 10.1371/journal.pntd.0004157.t002; 10.1371/journal.pntd.0004157.t004; 10.1371/journal.pntd.0004157.t003; 10.1371/journal.pntd.0004157.g001
PMC4618926; 4618926
Chanika Worasith; Christine Kamamia; Anna Yakovleva; Kunyarat Duenngai; Chompunoot Wangboon; Jiraporn Sithithaworn; Nattaya Watwiengkam; Nisana Namwat; Anchalee Techasen; Watcharin Loilome; Puangrat Yongvanit; Alex Loukas; Paiboon Sithithaworn; Jeffrey M. Bethony; Darren J. Gray Show More Hide
Public Library of Science (PLoS); Figshare
Medicine; Antigens; Helminth--urine; Opisthorchiasis--diagnosis; Opisthorchis--chemistry; Parasitology--methods; Urinalysis--methods; Biological Markers--analysis; Opisthorchiasis--diagnosis; Opisthorchiasis--pathology; Opisthorchis--pathogenicity; Proteinuria--diagnosis; Proteinuria--etiology; Uncategorised; positive predictive value; mif; ov infection; urine backgroundmany strategies; liver fluke infection; assay; coprological antigen detection methods; specific control methods; light ov infections; roc; regression.resultswhen urine samples; tca; es; opisthorchis viverrini antigen detection; mda; detection fect method.methodologywe; mass drug administration; lr; noninvasive sample collection; test result; Antigens, Helminth--urine; Opisthorchiasis--diagnosis; Opisthorchis--chemistry; Parasitology--methods; Urinalysis--methods; Immunology and Infectious Disease; Medical Immunology; Medical Microbiology; Parasitic Diseases; Parasitology; Biological Markers--analysis; Opisthorchiasis--pathology; Opisthorchis--pathogenicity; Proteinuria--diagnosis; Proteinuria--etiology
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Many strategies to control opisthorchiasis have been employed in Thailand, but not in the other neighbouring countries. Specific control methods include mass drug administration (MDA) and health education to reduce raw fish consumption. These control efforts have greatly shifted the epidemiology of Opisthorchis viverrini (OV) infection over the last decade from presenting as densely concentrated "heavy" infections in single villages to widespread "light" OV infections distributed over wide geographical areas. Currently, the "gold standard" detection method for OV infection is formalin ethyl-acetate concentration technique (FECT), which has limited diagnostic sensitivity and diagnostic specificity for light OV infections, with OV eggs often confused with eggs of minute intestinal flukes (MIFs) in feces. In this study, we developed and evaluated the diagnostic performance of a monoclonal antibody-based enzyme-linked immunosorbent assay for the measurement of OV excretory-secretory (ES) antigens in urine (urine OV-ES assay) for the diagnosis of opisthorchiasis compared to the gold standard detection FECT method.