ANG1 treatment reduces muscle pathology and prevents a decline in perfusion in DMD mice.

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PloS one, ISSN: 1932-6203, Vol: 12, Issue: 3, Page: e0174315

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10.1371/journal.pone.0174315; 10.1371/journal.pone.0174315.g001; 10.1371/journal.pone.0174315.g007; 10.1371/journal.pone.0174315.g004; 10.1371/journal.pone.0174315.g006; 10.1371/journal.pone.0174315.g005; 10.1371/journal.pone.0174315.t001; 10.1371/journal.pone.0174315.g003; 10.1371/journal.pone.0174315.g002
Kelly M. Gutpell; Nikola Tasevski; Boaz Wong; William Thomas Hrinivich; Feng Su; Jennifer Hadway; Lise Desjardins; Ting-Yim Lee; Lisa Marie Hoffman; Diego Fraidenraich
Public Library of Science (PLoS); Figshare
Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; ENDOTHELIAL GROWTH-FACTOR; DUCHENNE MUSCULAR-DYSTROPHY; SKELETAL-MUSCLE; VEGF OVEREXPRESSION; STEM-CELLS; MDX MICE; ANGIOPOIETIN-1; MODEL; ANGIOGENESIS; ISCHEMIA; Medicine; Cell Biology; Physiology; Biotechnology; Immunology; Cancer; Space Science; 69999 Biological Sciences not elsewhere classified; 19999 Mathematical Sciences not elsewhere classified; muscle tissue perfusion; dmd mice vascular; alpha-smooth muscle actin-positive vessels; vegf; ang 1; ang 1 treatment; pro-angiogenic growth factors; therapy; Anatomy; Cell and Developmental Biology
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Vascular endothelial growth factor (VEGF) and other pro-angiogenic growth factors have been investigated to enhance muscle tissue perfusion and repair in Duchenne muscular dystrophy (DMD). Current understanding is limited by a lack of functional data following in vivo delivery of these growth factors. We previously used dynamic contrast-enhanced computed tomography to monitor disease progression in murine models of DMD, but no study to date has utilized this imaging technique to assess vascular therapy in a preclinical model of DMD. In the current study, we locally delivered VEGF and ANG1 alone or in combination to dystrophic hind limb skeletal muscle. Using functional imaging, we found the combination treatment as well as ANG1 alone prevented decline in muscle perfusion whereas VEGF alone had no effect compared to controls. These findings were validated histologically as demonstrated by increased alpha-smooth muscle actin-positive vessels in muscles that received either VEGF+ANG1 or ANG1 alone compared to the sham group. We further show that ANG1 alone slows progression of fibrosis compared to either sham or VEGF treatment. The findings from this study shed new light on the functional effects of vascular therapy and suggest that ANG1 alone may be a candidate therapy in the treatment of DMD.