DsbA-L protein levels in white adipose tissue in an obesity model.

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Kerley, Madison E
obesity; DsbA-L; adiponectin; diabetes; obesity; DsbA-L; adiponectin; diabetes; Biology
thesis / dissertation description
Adiponectin is an adipose tissue-secreted protein found in three isoforms. Disulfide bond A oxidoreductase-like protein (DsbA-L) is thought to assist with protomeric disulfide bonding to generate the HMW (high molecular weight) isoform, which regulates insulin sensitivity. Both adiponectin and DsbA-L were previously found to negatively correlate with obesity.Immunoblots were conducted on adipose tissue samples from male mice fed a high or low-fat diet for 6, 10, or 16 weeks. Immunoblots from high-fat diet-fed mice revealed double bands for DsbA-L. High and low molecular weight bands were analyzed together, revealing significantly higher relative band densities with 10 and 16-week high-fat diet feedings compared to 6-week high-fat diet feedings, as well as with 10-week low-fat diet feedings compared to 6-week low-fat diet feedings. 6-week high-fat diet feedings showed higher relative band density than 6-week low-fat diet feedings. Results indicate significant DsbA-L expression upregulation with increased exposure time to a high-fat diet.