High Throughput Sequencing Identifies MicroRNAs Mediating α-Synuclein Toxicity by Targeting Neuroactive-Ligand Receptor Interaction Pathway in Early Stage of Drosophila Parkinson's Disease Model.

Citation data:

PloS one, ISSN: 1932-6203, Vol: 10, Issue: 9, Page: e0137432

Publication Year:
2015
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Repository URL:
https://jdc.jefferson.edu/medfp/134
PMID:
26361355
DOI:
10.1371/journal.pone.0137432; 10.1371/journal.pone.0137432.g006; 10.1371/journal.pone.0137432.t002; 10.1371/journal.pone.0137432.g002; 10.1371/journal.pone.0137432.g004; 10.1371/journal.pone.0137432.g001; 10.1371/journal.pone.0137432.g003; 10.1371/journal.pone.0137432.g009; 10.1371/journal.pone.0137432.t003; 10.1371/journal.pone.0137432.g005; 10.1371/journal.pone.0137432.t001; 10.1371/journal.pone.0137432.g008; 10.1371/journal.pone.0137432.g007
PMCID:
PMC4567341; 4567341
Author(s):
Kong, Yan; Liang, Xijun; Liu, Lin; Zhang, Dongdong; Wan, Chao; Gan, Zhenji; Yuan, Liudi; Jiang, Bing-Hua
Publisher(s):
Public Library of Science (PLoS); Figshare
Tags:
Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Thomas Jefferson University; animal cell; animal experiment; animal model; animal tissue; Article; controlled study; disease model; Drosophila; female; gene expression regulation; gene sequence; gene targeting; genetic damage; genetic identification; high throughput sequencing; in vivo study; male; neuropathology; neurotoxicity; nonhuman; Parkinson disease; pathogenesis; protein interaction; Uncategorised; diana; brain mirna expression; 30p; nmda; synuclein; gaba; receptor; lewy bodies; luciferase reporter assay; kegg pathway analysis; d 2r nmdar 2; rna sequencing technology; utr; drosophila pd model; Medical Molecular Biology
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Parkinson's disease (PD) is a prevalent neurodegenerative disorder with pathological features including death of dopaminergic neurons in the substantia nigra and intraneuronal accumulations of Lewy bodies. As the main component of Lewy bodies, α-synuclein is implicated in PD pathogenesis by aggregation into insoluble filaments. However, the detailed mechanisms underlying α-synuclein induced neurotoxicity in PD are still elusive. MicroRNAs are ~20nt small RNA molecules that fine-tune gene expression at posttranscriptional level. A plethora of miRNAs have been found to be dysregulated in the brain and blood cells of PD patients. Nevertheless, the detailed mechanisms and their in vivo functions in PD still need further investigation. By using Drosophila PD model expressing α-synuclein A30P, we examined brain miRNA expression with high-throughput small RNA sequencing technology. We found that five miRNAs (dme-miR-133-3p, dme-miR-137-3p, dme-miR-13b-3p, dme-miR-932-5p, dme-miR-1008-5p) were upregulated in PD flies. Among them, miR-13b, miR-133, miR-137 are brain enriched and highly conserved from Drosophila to humans. KEGG pathway analysis using DIANA miR-Path demonstrated that neuroactive-ligand receptor interaction pathway was most likely affected by these miRNAs. Interestingly, miR-137 was predicted to regulate most of the identified targets in this pathway, including dopamine receptor (DopR, D2R), γ-aminobutyric acid (GABA) receptor (GABA-B-R1, GABA-B-R3) and N-methyl-D-aspartate (NMDA) receptor (Nmdar2). The validation experiments showed that the expression of miR-137 and its targets was negatively correlated in PD flies. Further experiments using luciferase reporter assay confirmed that miR-137 could act on specific sites in 3' UTR region of D2R, Nmdar2 and GABA-B-R3, which downregulated significantly in PD flies. Collectively, our findings indicate that α-synuclein could induce the dysregulation of miRNAs, which target neuroactive ligand-receptor interaction pathway in vivo. We believe it will help us further understand the contribution of miRNAs to α-synuclein neurotoxicity and provide new insights into the pathogenesis driving PD.