Successful identification of pathogens by polymerase chain reaction (PCR)-based electron spray ionization time-of-flight mass spectrometry (ESI-TOF-MS) in culture-negative periprosthetic joint infection.

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The Journal of bone and joint surgery. American volume, ISSN: 1535-1386, Vol: 94, Issue: 24, Page: 2247-54

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Jacovides, Christina L; Kreft, Rachael; Adeli, Bahar; Hozack, Bryan; Ehrlich, Garth D; Parvizi, Javad
Ovid Technologies (Wolters Kluwer Health); Journal of Bone and Joint Surgery
Medicine; Rothman Institute of Orthopedics; Thomas Jefferson University Hospital; Aged; Anti-Bacterial Agents; Arthroplasty; Replacement; Hip; Knee; Chi-Square Distribution; Female; Humans; Male; Middle Aged; Polymerase Chain Reaction; Prospective Studies; Prosthesis-Related Infections; Reoperation; Sensitivity and Specificity; Spectrometry; Mass; Electrospray Ionization; Orthopedics
article description
The diagnosis of periprosthetic joint infection poses many challenges, one of which is the difficulty of isolating the infecting organism. Recently, a sophisticated modality (the Ibis Biosciences T5000 biosensor system) has been introduced that uses pan-domain primers in a series of polymerase chain reactions (PCRs) to identify and speciate essentially all bacteria and fungi as well as to identify key antibiotic resistance genes. We investigated the role of the Ibis in identifying infecting organisms in cases of known and suspected periprosthetic joint infection.