Determination of Caffeine Content in Brewed Caffeinated and Decaffeinated Coffee

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Chalmers, Logan
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Consumer curiosity has sparked the investigation of the caffeine content found in locally brewed Blackbird Coffee. A procedure involving liquid-liquid extraction with dichloromethane was developed to determine the amount of caffeine in brewed caffeinated and decaffeinated Starbuck's Dark Sumatra coffee. Recent adaptations to the procedure include a sodium hydroxide wash of the extracted organic layer. This has resulted in a change from a dark brown to a light yellow color of the extracted caffeine, indicating fewer impurities exist. The caffeine amounts recovered were reproducible, thus the method was proven to be reliable and used to determine the caffeine content of BlackBird coffee.