A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes.

Citation data:

Genetica, ISSN: 1573-6857, Vol: 139, Issue: 8, Page: 961-72

Publication Year:
2011
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Repository URL:
https://works.bepress.com/richard_hellmich/30; https://lib.dr.iastate.edu/ent_pubs/132
PMID:
21822602
DOI:
10.1007/s10709-011-9590-0
Author(s):
Coates, Brad S.; Sumerford, Douglas V.; Lopez, Miriam D.; Wang, Haichuan; Fraser, Lisa M.; Kroemer, Jeremy A.; Spencer, Terence; Kim, Kyung S.; Abel, Craig A.; Hellmich, Richard L.; Siegfried, Blair D. Show More Hide
Publisher(s):
Springer Nature
Tags:
Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Quantitative trait locus (QTL); Amplified fragment length polymorphism (AFLP); Single nucleotide polymorphism (SNP)
article description
The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae) and is capable of survival on transgenic hybrid corn. Genetic linkage maps with segregating AFLP markers show that the Cry1F resistance trait is controlled by a single quantitative trait locus (QTL) on linkage group 12. The map position of single nucleotide polymorphism (SNP) markers indicated that midgut Bt toxin-receptor genes, alkaline phosphatase, aminopeptidase N, and cadherin, are not linked with the Cry1F QTL. Evidence suggests that genes within this genome interval may give rise to a novel Bt toxin resistance trait for Lepidoptera that appears independent of known receptor-based mechanisms of resistance.