Hyphenated Applications of Mass Spectrometry for the Analysis of Biological Compounds

Publication Year:
2012
Usage 787
Downloads 758
Abstract Views 29
Repository URL:
https://lib.dr.iastate.edu/etd/12863
DOI:
10.31274/etd-180810-121
Author(s):
Morast, Derrick
Publisher(s):
Iowa State University; Digital Repository @ Iowa State University
Tags:
APPI; Ion Mobility; Laser Desportion; Mass Spectrometry; Phosvitin; Zein
thesis / dissertation description
A variety of hyphenated mass spectrometry techniques were implemented for the analysis of biological compounds. Ion mobility combined with mass spectrometry was used to measure gas-phase conformations of proteins sprayed from slightly alkaline solutions. High-resolution mass spectrometry (HRMS) was utilized for a comprehensive analysis of alpha-zein proteins extracted from corn gluten meal (CGM) and distillers' dried grains with solubles (DDGS). Electrospray ionization (ESI) and tandem mass spectrometry were used to characterize phosphopeptides from the egg yolk protein phosvitin. And finally, a novel atmospheric pressure ionization source which combined UV laser desorption and atmospheric pressure photoionization was introduced.