The fragile Fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components.

Citation data:

Plant physiology, ISSN: 1532-2548, Vol: 167, Issue: 3, Page: 780-92

Publication Year:
2015
Usage 79
Abstract Views 45
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Citations 36
Citation Indexes 36
Repository URL:
https://openscholarship.wustl.edu/bio_facpubs/80
PMID:
25646318
DOI:
10.1104/pp.114.251462
PMCID:
PMC4348757
Author(s):
Zhu, Chuanmei; Ganguly, Anindya; Baskin, Tobias I; McClosky, Daniel D; Anderson, Charles T; Foster, Cliff; Meunier, Kristoffer A; Okamoto, Ruth; Berg, Howard; Dixit, Ram
Publisher(s):
American Society of Plant Biologists (ASPB)
Tags:
Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; kinesin; FRA1; Microtubule proteins; Biochemistry; Biology; Plant Biology
article description
The cell wall consists of cellulose microfibrils embedded within a matrix of hemicellulose and pectin. Cellulose microfibrils are synthesized at the plasma membrane, whereas matrix polysaccharides are synthesized in the Golgi apparatus and secreted. The trafficking of vesicles containing cell wall components is thought to depend on actin-myosin. Here, we implicate microtubules in this process through studies of the kinesin-4 family member, Fragile Fiber1 (FRA1). In an fra1-5 knockout mutant, the expansion rate of the inflorescence stem is halved compared with the wild type along with the thickness of both primary and secondary cell walls. Nevertheless, cell walls in fra1-5 have an essentially unaltered composition and ultrastructure. A functional triple green fluorescent protein-tagged FRA1 fusion protein moves processively along cortical microtubules, and its abundance and motile density correlate with growth rate. Motility of FRA1 and cellulose synthase complexes is independent, indicating that FRA1 is not directly involved in cellulose biosynthesis; however, the secretion rate of fucose-alkyne-labeled pectin is greatly decreased in fra1-5, and the mutant has Golgi bodies with fewer cisternae and enlarged vesicles. Based on our results, we propose that FRA1 contributes to cell wall production by transporting Golgi-derived vesicles along cortical microtubules for secretion.