Phase imaging digital holography for biological microscopy

Publication Year:
2004
Usage 373
Downloads 350
Abstract Views 23
Repository URL:
http://scholarcommons.usf.edu/etd/1191
Author(s):
Parshall, Daniel
Tags:
microscope; cells; optics; biophysics; hologram
thesis / dissertation description
We apply the techniques of digital holography to obtain microscopic 3D images of biological cells. Both the amplitude and phase images are obtained from a single hologram, with approximately 1 [mu]m lateral and 10 nm longitudinal resolution. The results are combined with previous experiments resolving the 2 [pi] ambiguity to produce continuous phase images of the samples.