Microarray analysis of gene expression profiles of tetrahymena H2B ubiquitylation mutant strains

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Wang, Zhe
H2B; Microarray; Tetrahymena; Ubiquitylation
thesis / dissertation description
Microarray analyses were performed to compare the gene expression profiles of wild-type and mutant strains of Tetrahymena thermophila. These studies suggest that, in Tetrahymena, ubiquitylation of histone H2B is not required for H3K4 methylation. Even though the E2 ubiquitin-conjugating enzyme Ubc2 and the E3 ubiquitin ligase Bre1 are required for H2B ubiquitylation, these two enzymes affect the expression of a much broader spectrum of genes than H2B ubiquitylation, suggesting that Ubc2 and Bre1 are involved in pathways other than H2B ubiquitylation in Tetrahymena.