The Adaptability of Several Histological Techniques to the Preparation of P32 Radioautographs from Plant Tissues

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Proceedings of the Iowa Academy of Science, Vol: 57, Issue: 1, Page: 135-140

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McIlrath, Wayne J.
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With the increased use of radioactive isotopes in plant research, there has been a corresponding increase in the application of radioautographic techniques. Through the use of such radioautographs, tissue localization of the radioactive elements has been accomplished thereby giving additional insight into the accumulation and role of various elements in specific tissues. One of the radioactive elements that has found widespread application in physiological research is P 32. Although a number of workers have prepared P32 autographs from unsectioned plant tissues, very few have been made using histological sections (McIlrath, 1948). Because of the widespread use of this radioactive element in plant research and the very few histological radioautographs found in the literature, this problem was undertaken to determine which of several histological techniques might be adaptable to the preparation of such radioautographs.