20-Hydroxyecdysone (20E) Primary Response Gene E75 Isoforms Mediate Steroidogenesis Autoregulation and Regulate Developmental Timing in Bombyx.

Citation data:

The Journal of biological chemistry, ISSN: 1083-351X, Vol: 291, Issue: 35, Page: 18163-75

Publication Year:
2016
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Repository URL:
https://uknowledge.uky.edu/entomology_facpub/122
PMID:
27365399
DOI:
10.1074/jbc.m116.737072
PMCID:
PMC5000065
Author(s):
Li, Kang; Tian, Ling; Guo, Zhongjian; Guo, Sanyou; Zhang, Jianzhen; Gu, Shi-Hong; Palli, Subba Reddy; Cao, Yang; Li, Sheng
Publisher(s):
American Society for Biochemistry & Molecular Biology (ASBMB); American Society for Biochemistry and Molecular Biology
Tags:
Biochemistry, Genetics and Molecular Biology; development; nuclear receptor; protein-protein interaction; signal transduction; steroidogenesis; Halloween genes; ROREs; feedback regulation; isoform-specific; Biochemistry, Biophysics, and Structural Biology; Entomology
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article description
The temporal control mechanisms that precisely control animal development remain largely elusive. The timing of major developmental transitions in insects, including molting and metamorphosis, is coordinated by the steroid hormone 20-hydroxyecdysone (20E). 20E involves feedback loops to maintain pulses of ecdysteroid biosynthesis leading to its upsurge, whereas the underpinning molecular mechanisms are not well understood. Using the silkworm Bombyx mori as a model, we demonstrated that E75, the 20E primary response gene, mediates a regulatory loop between ecdysteroid biosynthesis and 20E signaling. E75 isoforms A and C directly bind to retinoic acid receptor-related response elements in Halloween gene promoter regions to induce gene expression thus promoting ecdysteroid biosynthesis and developmental transition, whereas isoform B antagonizes the transcriptional activity of isoform A/C through physical interaction. As the expression of E75 isoforms is differentially induced by 20E, the E75-mediated regulatory loop represents a fine autoregulation of steroidogenesis, which contributes to the precise control of developmental timing.