Pyrosequencing the Bemisia tabaci transcriptome reveals a highly diverse bacterial community and a robust system for insecticide resistance.

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PloS one, ISSN: 1932-6203, Vol: 7, Issue: 4, Page: e35181

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10.1371/journal.pone.0035181; 10.1371/journal.pone.0035181.g006; 10.1371/journal.pone.0035181.t003; 10.1371/journal.pone.0035181.g003; 10.1371/journal.pone.0035181.t001; 10.1371/journal.pone.0035181.g002; 10.1371/journal.pone.0035181.g004; 10.1371/journal.pone.0035181.g005; 10.1371/journal.pone.0035181.t002; 10.1371/journal.pone.0035181.t004; 10.1371/journal.pone.0035181.t005; 10.1371/journal.pone.0035181.g001
PMC3340392; 3340392
Wen Xie; Qing-shu Meng; Qing-jun Wu; Shao-li Wang; Xin Yang; Ni-na Yang; Ru-mei Li; Xiao-guo Jiao; Hui-peng Pan; Bai-ming Liu; Qi Su; Bao-yun Xu; Song-nian Hu; Xu-guo Zhou; You-jun Zhang; John Vontas Show More Hide
Public Library of Science (PLoS); Figshare
Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Animals; Bacteria; Base Sequence; Computational Biology; Expressed Sequence Tags; Gene Expression Profiling; Gene Expression Regulation; Gene Library; Hemiptera; Host-Pathogen Interactions; Insecticide Resistance; Metagenomics; Molecular Sequence Annotation; Molecular Sequence Data; Nitro Compounds; Oxazines; Phylogeny; Real-Time Polymerase Chain Reaction; Sequence Analysis; DNA; Symbiosis; Thiazoles; Transcriptome; Microbiology; Genetics; Biotechnology; whitefly; microsatellite; loci; genetics and genomics; microbiology; biotechnology; Entomology; hits; sequences; phylogeny; bacterial; symbionts; unigenes; ontology; p450s; amino-acid; biosynthetic; pathways; profiling; putative; thiamethoxam; resistant; susceptible; pyrosequencing; transcriptome; reveals; robust; insecticide; classification; symbiotic
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Bemisia tabaci (Gennadius) is a phloem-feeding insect poised to become one of the major insect pests in open field and greenhouse production systems throughout the world. The high level of resistance to insecticides is a main factor that hinders continued use of insecticides for suppression of B. tabaci. Despite its prevalence, little is known about B. tabaci at the genome level. To fill this gap, an invasive B. tabaci B biotype was subjected to pyrosequencing-based transcriptome analysis to identify genes and gene networks putatively involved in various physiological and toxicological processes.