Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR
Revista da Sociedade Brasileira de Medicina Tropical, ISSN: 0037-8682, Vol: 51, Issue: 4, Page: 528-532
2018
- 8Citations
- 2,686Usage
- 19Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations8
- Citation Indexes8
- CrossRef1
- Usage2,686
- Full Text Views2,608
- 2,608
- Abstract Views78
- 78
- Captures19
- Readers19
- 19
Article Description
Introduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85052052652&origin=inward; http://dx.doi.org/10.1590/0037-8682-0352-2017; http://www.ncbi.nlm.nih.gov/pubmed/30133639; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400528&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0037-86822018000400528&lng=en&tlng=en; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400528; http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0037-86822018000400528; https://dx.doi.org/10.1590/0037-8682-0352-2017; https://www.scielo.br/j/rsbmt/a/J94rdwPChyz4MVz8x9vRbPR/?lang=en
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