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Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR

Revista da Sociedade Brasileira de Medicina Tropical, ISSN: 0037-8682, Vol: 51, Issue: 4, Page: 528-532
2018
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Metric Options:   Counts1 Year3 Year

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Article Description

Introduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.

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