PlumX Metrics
Embed PlumX Metrics

Real-time polymerase chain reaction for detection and enumeration of Staphylococcus aureus and Streptococccus agalactiae using different milk samplings

Revista Brasileira de Zootecnia, ISSN: 1806-9290, Vol: 52
2023
  • 1
    Citations
  • 0
    Usage
  • 6
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    1
  • Captures
    6
  • Mentions
    1
    • News Mentions
      1
      • 1

Most Recent News

Researchers from University of Sao Paulo Detail New Studies and Findings in the Area of Staphylococcus aureus (Real-time Polymerase Chain Reaction for Detection and Enumeration of Staphylococcus Aureus and Streptococccus Agalactiae Using ...)

2023 MAY 16 (NewsRx) -- By a News Reporter-Staff News Editor at NewsRx Life Science Daily -- Investigators publish new report on Gram-Positive Bacteria -

Article Description

The objective of the present study was to evaluate the qPCR for detection and enumeration of Staphylococcus aureus and Streptococcus agalactiae using different milk samplings in comparison to the conventional microbiology. Four dairy herds with a history of subclinical mastitis caused by S. aureus and S. agalactiae were selected. Sampling approach included milk samples from bulk tank (BT), cow level (composite samples, CO), and mammary quarter level (MQ) from 785 lactating cows. Three consecutive monthly milk samplings were carried out, totaling 3347 MQ milk samples, 912 CO, and 12 from BT. All collected milk samples were subjected to conventional microbiology and qPCR for detection and enumeration of S. aureus and S. agalactiae. The qPCR showed 71.5% of diagnostic sensitivity for S. aureus isolated from MQ milk samples, 71.8% for CO, and 50% for BT milk samples compared with conventional microbiology methodology. Taken together, the diagnostic sensitivity for S. agalactiae isolated from MQ milk samples was 90.2, 87.7 for CO, and 90.9% for BT milk samples. In general, the qPCR methodology enabled the detection of S. aureus and S. agalactiae, regardless of the type of milk sampling. The direct use of milk samples to estimate the counting of S. aureus by qPCR demonstrated lower sensitivity than the counting of S. agalactiae, which can be explained by the pathogen infection dynamics and differences in milk sample type

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know