HPLC determination of α-ketoglutaramate [5-amino-2,5-dioxopentanoate] in biological samples.

Citation data:

Analytical biochemistry, ISSN: 1096-0309, Vol: 494, Page: 52-4

Publication Year:
2016
Usage 55
Abstract Views 51
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Repository URL:
https://touroscholar.touro.edu/nymc_fac_pubs/53; https://dx.doi.org/10.1016/j.ab.2015.11.003
PMID:
26576832
DOI:
10.1016/j.ab.2015.11.003
Author(s):
Shurubor, Yevgeniya I; Cooper, Arthur J. L.; Isakova, Elena P; Deryabina, Yulia I; Beal, M Flint; Krasnikov, Boris F
Publisher(s):
Elsevier BV; Academic Press; Touro Scholar
Tags:
Biochemistry, Genetics and Molecular Biology; Animals; Brain; Chemistry Techniques; Analytical; Chromatography; High Pressure Liquid; Ketoglutaric Acids; Kidney; Liver; Rats; Chemistry Techniques, Analytical; Chromatography, High Pressure Liquid; Biochemistry; Molecular Biology
article description
α-Ketoglutaramate is an important glutamine metabolite in mammals, plants, and many bacteria. It is also a nicotine metabolite in certain bacteria. Previously published methods for the determination of α-ketoglutaramate in biological samples have considerable drawbacks. Here, we describe a relatively simple high-performance liquid chromatography (HPLC)-based method for measurement of α-ketoglutaramate in plasma and deproteinized tissues that overcomes these drawbacks. Concentrations of α-ketoglutaramate in normal rat liver, kidney, brain, and plasma were found to be approximately 216, 13, 6, and 19 μM, respectively. The HPLC method should be useful for studying the role of α-ketoglutaramate in eukaryotic glutamine metabolism and in bacterial nicotine metabolism.