Metabolic engineering of Klebsiella pneumoniae J2B for co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol: Reduction of acetate and other by-products.

Citation data:

Bioresource technology, ISSN: 1873-2976, Vol: 244, Issue: Pt 1, Page: 1096-1103

Publication Year:
Usage 7
Abstract Views 6
Link-outs 1
Social Media 1
Tweets 1
Citations 10
Citation Indexes 10
Repository URL:
Ko, Yeounjoo; Seol, Eunhee; Sundara Sekar, Balaji; Kwon, Seongjin; Lee, Jaehyeon; Park, Sunghoon
Elsevier BV
Chemical Engineering; Environmental Science; Energy; Co-production; 3-Hydroxypropionic acid; 1,3-Propanediol; Klebsiella pneumoniae; Acetate reduction
Most Recent Tweet View All Tweets
article description
Production of 3-hydroxypropionic acid (3-HP) or 1,3-propanediol (1,3-PDO) production from glycerol is challenging due to the problems associated with cofactor regeneration, coenzyme B synthesis, and the instability of pathway enzymes. To address these complications, simultaneous production of 3-HP and 1,3-PDO, instead of individual production of each compound, was attempted. With over-expression of an aldehyde dehydrogenase, recombinant Klebsiella pneumoniae could co-produce 3-HP and 1,3-PDO successfully. However, the production level was unsatisfactory due to excessive accumulation of many by-products, especially acetate. To reduce acetate production, we attempted; (i) reduction of glycerol assimilation through the glycolytic pathway, (ii) increase of glycerol flow towards co-production, and (iii) variation of aeration rate. These efforts were partially beneficial in reducing acetate and improving co-production: 21g/L of 1,3-PDO and 43g/L of 3-HP were obtained. Excessive acetate (>150mM) was still produced at the end of bioreactor runs, and limited co-production efficiency.