Femtosecond laser-induced dissociation (fs-LID) as an activation method in mass spectrometry

Citation data:

Chemical Physics, ISSN: 0301-0104, Vol: 514, Page: 106-112

Publication Year:
2018
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Readers 2
Repository URL:
https://www.sciencedirect.com/science/article/pii/S0301010417309989
DOI:
10.1016/j.chemphys.2018.05.008
Author(s):
Christian Neidel; Andreas Kuehn; Claus Peter Schulz; Ingolf V. Hertel; Michael W. Linscheid; Thomas Schultz
Publisher(s):
Elsevier BV
Tags:
Physics and Astronomy; Chemistry; Fs-LID; Peptides; Proteins; Sequencing; Mass spectrometry; Femtosecond laser; FT-MS
article description
We report the photolysis of biomolecules in a Fourier-transform ion cyclotron resonance mass spectrometer by intense near-infrared femtosecond laser pulses. Photo-fragmentation was accompanied by photo-ionization and created a large number and variety of charged fragments, which could be identified with high confidence due to the outstanding resolving power and mass accuracy of the mass spectrometer. Fragmentation patterns were sufficient for peptide sequence analysis. Fragments formed by non-ergodic cleavage retained labile post-translational modifications.