Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of sp. and sp. through bioprocess optimization.
- Citation data:
Synthetic and systems biotechnology, ISSN: 2405-805X, Vol: 3, Issue: 2, Page: 121-129
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- Biochemistry, Genetics and Molecular Biology; Engineering; Immunology and Microbiology; Thraustochytrids; Glycerol; Polyunsaturated fatty acids; Docosahexaenoic acids; Batch culture
Thraustochytrids, rich in docosahexaenoic acid (DHA, C22:6ω3), represent a potential source of dietary fatty acids. Yet, the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains. Two different thraustochytrid strains, sp. PKU#Mn4 and sp. PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions. Although they exhibited similar fatty acid profiles, PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions. Relative DHA content of 48.5% and 49.2% (relative to total fatty acids), respectively, were achieved on glycerol under their optimal flask culture conditions. Maximum DHA yield (Y) of 21.0% and 18.9% and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained, respectively, in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy. A 3.4- and 2.8-fold improvement of DHA production (g/L), respectively, was achieved in this study. Overall, our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil.