Large-field-of-view imaging by multi-pupil adaptive optics.

Citation data:

Nature methods, ISSN: 1548-7105, Vol: 14, Issue: 6, Page: 581-583

Publication Year:
2017
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/21983
PMID:
28481364
DOI:
10.1038/nmeth.4290
Author(s):
Park, Jung-Hoon; Kong, Lingjie; Zhou, Yifeng; Cui, Meng
Publisher(s):
Springer Nature; NATURE PUBLISHING GROUP
Tags:
Biochemistry, Genetics and Molecular Biology; Fluorescence imaging; Mouse; Neuroscience; Optical imaging
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article description
Adaptive optics can correct for optical aberrations. We developed multi-pupil adaptive optics (MPAO), which enables simultaneous wavefront correction over a field of view of 450 × 450 μm and expands the correction area to nine times that of conventional methods. MPAO's ability to perform spatially independent wavefront control further enables 3D nonplanar imaging. We applied MPAO to in vivo structural and functional imaging in the mouse brain.