Large-field-of-view imaging by multi-pupil adaptive optics.
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Nature methods, ISSN: 1548-7105, Vol: 14, Issue: 6, Page: 581-583
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- Biochemistry, Genetics and Molecular Biology, Fluorescence imaging, Mouse, Neuroscience, Optical imaging
Adaptive optics can correct for optical aberrations. We developed multi-pupil adaptive optics (MPAO), which enables simultaneous wavefront correction over a field of view of 450 × 450 μm and expands the correction area to nine times that of conventional methods. MPAO's ability to perform spatially independent wavefront control further enables 3D nonplanar imaging. We applied MPAO to in vivo structural and functional imaging in the mouse brain.