Single-molecule nanometry for biological physics.

Citation data:

Reports on progress in physics. Physical Society (Great Britain), ISSN: 1361-6633, Vol: 76, Issue: 1, Page: 016601

Publication Year:
2013
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Citations 20
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Repository URL:
http://iopscience.iop.org/0034-4885/76/1/016601/
PMID:
23249673
DOI:
10.1088/0034-4885/76/1/016601
PMCID:
PMC3549428
Author(s):
Kim, Hajin; Ha, Taekjip
Publisher(s):
IOP Publishing
Tags:
Physics and Astronomy
review description
Precision measurement is a hallmark of physics but the small length scale (∼nanometer) of elementary biological components and thermal fluctuations surrounding them challenge our ability to visualize their action. Here, we highlight the recent developments in single-molecule nanometry where the position of a single fluorescent molecule can be determined with nanometer precision, reaching the limit imposed by the shot noise, and the relative motion between two molecules can be determined with ∼0.3 nm precision at ∼1 ms time resolution, as well as how these new tools are providing fundamental insights into how motor proteins move on cellular highways. We will also discuss how interactions between three and four fluorescent molecules can be used to measure three and six coordinates, respectively, allowing us to correlate the movements of multiple components. Finally, we will discuss recent progress in combining angstrom-precision optical tweezers with single-molecule fluorescent detection, opening new windows for multi-dimensional single-molecule nanometry for biological physics.