Feasibility of a facile butanol bioproduction using planetary mill pretreatment.

Citation data:

Bioresource technology, ISSN: 1873-2976, Vol: 199, Page: 283-287

Publication Year:
2016
Usage 59
Abstract Views 59
Captures 21
Readers 21
Citations 4
Citation Indexes 4
Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/18095
PMID:
26372608
DOI:
10.1016/j.biortech.2015.08.074
Author(s):
Kwon, Jeong Heo; Kang, Hyunsoo; Sang, Byoung-In; Kim, Yunje; Min, Jiho; Mitchell, Robert J.; Lee, Jin Hyung
Publisher(s):
Elsevier BV; ELSEVIER SCI LTD
Tags:
Chemical Engineering; Environmental Science; Energy; Planetary mill; Pretreatment; Enhanced bioconversion; Biobutanol; Biofuel
article description
A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 ± 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91 and 6.66 g/L, respectively.