Metabolic engineering of Klebsiella pneumoniae J2B for the production of 1,3-propanediol from glucose.

Citation data:

Bioresource technology, ISSN: 1873-2976, Vol: 245, Issue: Pt B, Page: 1542-1550

Publication Year:
2017
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/22864
PMID:
28549809
DOI:
10.1016/j.biortech.2017.05.052
Author(s):
Lama, Suman; Seol, Eunhee; Park, Sunghoon
Publisher(s):
Elsevier BV; ELSEVIER SCI LTD
Tags:
Chemical Engineering; Environmental Science; Energy; Klebsiella pneumoniae J2B; Glucose; Glycerol; 1,3-PDO
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article description
The production of 1,3-propanediol (1,3-PDO) from glucose was investigated using Klebsiella pneumoniae J2B, which converts glycerol to 1,3-PDO and synthesize an essential coenzyme B. In order to connect the glycolytic pathway with the pathway of 1,3-PDO synthesis from glycerol, i.e., to directly produce diol from glucose, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate phosphatase from Saccharomyces cerevisiae were overexpressed. Additionally, the effect of expression levels and the use of isoforms of these two enzymes on glycerol and 1,3-PDO production were studied. Furthermore, to prevent loss of produced glycerol, the glycerol oxidation pathways were disrupted. Finally, the conversion rate of glycerol to 1,3-PDO was increased via homologous overexpression of glycerol dehydratase and 1,3-PDO oxidoreductase. The resultant strain successfully produced 1,3-PDO from glucose at a yield of 0.27mol/mol along with glycerol at 0.52mol/mol. Improvement of the engineered K. pneumoniae J2B to further increase conversion of glycerol to 1,3-PDO is discussed.