Rapid and Automated Quantification of Microalgal Lipids on a Spinning Disc.
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Analytical chemistry, ISSN: 1520-6882, Vol: 87, Issue: 15, Page: 7865-71
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- Chemistry; ON-A-DISC; CHLAMYDOMONAS-REINHARDTII; MICROFLUIDIC PLATFORM; EXTRACTION; LAB; BIODIESEL; GROWTH; ACCUMULATION; DEPRIVATION; NITROGEN
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We have developed a fully integrated centrifugal microfluidic device for rapid on-site quantification of lipids from microalgal samples. The fully automated serial process involving cell sedimentation and lysis, liquid-liquid extraction, and colorimetric detection of lipid contents was accomplished within 13 min using a lab-on-a-disc. The presented organic solvent-tolerable (for n-hexane, ethanol) microfluidic disc was newly fabricated by combining thermal fusion bonding and carbon dot-based valving techniques. It is expected that this novel platform will possibly contribute toward sustainable biofuel applications by providing a practical solution for on-site monitoring of lipid accumulation in microalgal samples, thus providing imperative contribution toward energy and environmental purposes of centrifugal microfluidic technology.