Phosphorescent sensor for phosphorylated peptides based on an iridium complex.

Citation data:

The Journal of organic chemistry, ISSN: 1520-6904, Vol: 79, Issue: 13, Page: 6000-5

Publication Year:
Usage 11
Abstract Views 11
Captures 13
Readers 12
Exports-Saves 1
Citations 10
Citation Indexes 10
Repository URL:
Kang, Jung Hyun; Kim, Hee Jin; Kwon, Tae-Hyuk; Hong, Jong-In
American Chemical Society (ACS); AMER CHEMICAL SOC
Chemistry; Adenosinetriphosphate; Fluorescence; Iridium compounds; Light emission; Negative ions; Peptides; Phosphorescence; Phosphorylation; Zinc Cellular mechanisms; Emission intensity; Fluorescent sensors; Iridium complex; Metal-to-ligand charge transfer; Negative charge; Phosphorylated peptides; Self-quenching
article description
A bis[(4,6-difluorophenyl)pyridinato-N,C(2')]iridium(III) picolinate (FIrpic) derivative coupled with bis(Zn(2+)-dipicolylamine) (ZnDPA) was developed as a sensor (1) for phosphorylated peptides, which are related to many cellular mechanisms. As a control, a fluorescent sensor (2) based on anthracene coupled to ZnDPA was also prepared. When the total negative charge on the phosphorylated peptides was changed to -2, -4, and -6, the emission intensity of sensor 1 gradually increased by factors of up to 7, 11, and 16, respectively. In contrast, there was little change in the emission intensity of sensor 1 upon the addition of a neutral phosphorylated peptide, non-phosphorylated peptides, or various anions such as CO3(2-), NO3(-), SO4(2-), phosphate, azide, and pyrophosphate. Furthermore, sensor 1 could be used to visually discriminate between phosphorylated peptides and adenosine triphosphate in aqueous solution under a UV-vis lamp, unlike fluorescent sensor 2. This enhanced luminance of phosphorescent sensor 1 upon binding to a phosphorylated peptide is attributed to a reduction in the repulsion between the Zn(2+) ions due to the phenoxy anion, its strong metal-to-ligand charge transfer character, and a reduction in self-quenching.