An improved surface passivation method for single-molecule studies.

Citation data:

Nature methods, ISSN: 1548-7105, Vol: 11, Issue: 12, Page: 1233-6

Publication Year:
2014
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/9681
PMID:
25306544
DOI:
10.1038/nmeth.3143
PMCID:
PMC4245390
Author(s):
Hua, Boyang; Han, Kyu Young; Zhou, Ruobo; Kim, Hajin; Shi, Xinghua; Abeysirigunawardena, Sanjaya C.; Jain, Ankur; Singh, Digvijay; Aggarwal, Vasudha; Woodson, Sarah A.; Ha, Taek Jip Show More Hide
Publisher(s):
Springer Nature; NATURE PUBLISHING GROUP
Tags:
Biochemistry, Genetics and Molecular Biology; IN-VITRO; PROTEINS; HELICASE; DNA; INITIATION; DYNAMICS; COMPLEX; MYOSIN
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article description
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.