An improved surface passivation method for single-molecule studies.
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Nature methods, ISSN: 1548-7105, Vol: 11, Issue: 12, Page: 1233-6
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- Biochemistry, Genetics and Molecular Biology; IN-VITRO; PROTEINS; HELICASE; DNA; INITIATION; DYNAMICS; COMPLEX; MYOSIN
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We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.