Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics.

Citation data:

The Analyst, ISSN: 1364-5528, Vol: 141, Issue: 2, Page: 371-81

Publication Year:
2016
Usage 19
Abstract Views 18
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Citations 20
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/18166
PMID:
26535415
DOI:
10.1039/c5an01775k
Author(s):
Sunkara, Vijaya; Woo, Hyun-Kyung; Cho, Yoon-Kyoung
Publisher(s):
Royal Society of Chemistry (RSC); ROYAL SOC CHEMISTRY; The Royal Society of Chemistry
Tags:
Chemistry; Biochemistry, Genetics and Molecular Biology; Environmental Science; NANOPARTICLE TRACKING ANALYSIS; CELL-DERIVED EXOSOMES; PANCREATIC-CANCER; CIRCULATING EXOSOMES; FLOW-CYTOMETRY; INTERCELLULAR COMMUNICATION; HUMAN PLASMA; SERUM; BIOMARKER; MICROVESICLES
review description
Extracellular vesicles (EVs) are cell-derived nanovesicles, present in almost all types of body fluids, which play an important role in intercellular communication and are involved in the transport of biological signals for regulating diverse cellular functions. Due to the increasing clinical interest in the role of EVs in tumor promotion, various techniques for their isolation, detection, and characterization are being developed. In this review, we present an overview of the current EV isolation and characterization methods in addition to their applications and limitations. Furthermore, EVs as the potential emerging biomarkers in cancer management and their clinical implementation are briefly discussed.