An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier.
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Chemical communications (Cambridge, England), ISSN: 1364-548X, Vol: 51, Issue: 54, Page: 10945-8
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- Chemical Engineering; Materials Science; Chemistry; CATALYZED REPORTER DEPOSITION; CAGE NANOPARTICLES; CANCER-CELLS; AMPLIFICATION; NANOPLATFORM; EXPRESSION; PLATFORMS; DELIVERY; THERAPY
A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.