An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier.

Citation data:

Chemical communications (Cambridge, England), ISSN: 1364-548X, Vol: 51, Issue: 54, Page: 10945-8

Publication Year:
2015
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Citations 6
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Repository URL:
http://scholarworks.unist.ac.kr/handle/201301/12042
PMID:
26063640
DOI:
10.1039/c5cc02409a
Author(s):
Lee, Jisu; Song, Eun Kyung; Bae, Yoonji; Min, Junseon; Rhee, Hyun-Woo; Park, Tae Joo; Kim, Moonil; Kang, Sebyung
Publisher(s):
Royal Society of Chemistry (RSC); The Royal Society of Chemistry; ROYAL SOC CHEMISTRY
Tags:
Chemical Engineering; Materials Science; Chemistry; CATALYZED REPORTER DEPOSITION; CAGE NANOPARTICLES; CANCER-CELLS; AMPLIFICATION; NANOPLATFORM; EXPRESSION; PLATFORMS; DELIVERY; THERAPY
article description
A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.