Lab-on-a-disc for simultaneous determination of total phenolic content and antioxidant activity of beverage samples.

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Lab on a chip, ISSN: 1473-0189, Vol: 16, Issue: 17, Page: 3268-75

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Phonchai, Apichai; Kim, Yubin; Chantiwas, Rattikan; Cho, Yoon-Kyoung
Royal Society of Chemistry (RSC); The Royal Society of Chemistry; ROYAL SOC CHEMISTRY
Chemical Engineering; Biochemistry, Genetics and Molecular Biology; Chemistry; Engineering
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In this paper, we present a fully integrated and automated lab-on-a-disc for the rapid determination of the total phenolic content (TPC) and antioxidant activity (AA) of beverage samples. The simultaneous determinations of TPC and AA on a spinning disc were achieved by integrating three independent analytical techniques: the Folin-Ciocalteu method that is used to measure TPC, the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) method and the ferric reducing antioxidant power method that are used to measure AA. The TPC and AA of 8 different beverage samples, including various fruit juices, tea, wine and beer, were analyzed. Unlike conventional labor-intensive processes for measuring TPC and AA, our fully automated platform offers one-step operation and rapid analysis.