Multiple cell death pathways are independently activated by lethal hypertonicity in renal epithelial cells.

Citation data:

American journal of physiology. Cell physiology, ISSN: 1522-1563, Vol: 305, Issue: 10, Page: C1011-20

Publication Year:
Usage 22
Abstract Views 17
Link-outs 5
Captures 16
Readers 13
Exports-Saves 3
Citations 4
Citation Indexes 4
Repository URL:
Choi, Soo Youn; Lee-Kwon, Whaseon; Lee, Hwan Hee; Lee, Jun Ho; Sanada, Satoru; Kwon, Hyug Moo
American Physiological Society; AMER PHYSIOLOGICAL SOC
Biochemistry, Genetics and Molecular Biology; Caspase; Cathepsin B; Cytochrome c
article description
When hypertonicity is imposed with sufficient intensity and acuteness, cells die. Here we investigated the cellular pathways involved in death using a cell line derived from renal epithelium. We found that hypertonicity rapidly induced activation of an intrinsic cell death pathway-release of cytochrome c and activation of caspase-3 and caspase-9-and an extrinsic pathway-activation of caspase-8. Likewise, a lysosomal pathway of cell death characterized by partial lysosomal rupture and release of cathepsin B from lysosomes to the cytosol was also activated. Relationships among the pathways were examined using specific inhibitors. Caspase inhibitors did not affect cathepsin B release into the cytosol by hypertonicity. In addition, cathepsin B inhibitors and caspase inhibitors did not affect hypertonicity-induced cytochrome c release, suggesting that the three pathways were independently activated. Combined inhibition of caspases and cathepsin B conferred significantly more protection from hypertonicity-induced cell death than inhibition of caspase or cathepsin B alone, indicating that all the three pathways contributed to the hypertonicity-induced cell death. Similar pattern of sensitivity to the inhibitors was observed in two other cell lines derived from renal epithelia. We conclude that multiple cell death pathways are independently activated early in response to lethal hypertonic stress in renal epithelial cells.