Fabrication and Characterization of Collagen-Immobilized Porous PHBV/HA Nanocomposite Scaffolds for Bone Tissue Engineering
- Citation data:
Journal of Nanomaterials, ISSN: 1687-4110, Vol: 2012, Page: 1-11
- Publication Year:
- Repository URL:
- https://doi.org/10.1155/2012/171804; http://scholarworks.unist.ac.kr/handle/201301/16945
- Materials Science; NANOFIBROUS SCAFFOLDS; SURFACE MODIFICATION; OSTEOBLAST ADHESION; CELL ATTACHMENT; IN-VITRO; COMPOSITE; HYDROXYAPATITE; PROLIFERATION; POLYESTERS; MATRICES
The porous composite scaffolds (PHBV/HA) consisting of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and hydroxyapatite (HA) were fabricated using a hot-press machine and salt-leaching. Collagen (type I) was then immobilized on the surface of the porous PHBV/HA composite scaffolds to improve tissue compatibility. The structure and morphology of the collagen-immobilized composite scaffolds (PHBV/HA/Col) were investigated using a scanning electron microscope (SEM), Fourier transform infrared (FTIR), and electron spectroscopy for chemical analysis (ESCA). The potential of the porous PHBV/HA/Col composite scaffolds for use as a bone scaffold was assessed by an experiment with osteoblast cells (MC3T3-E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the PHBV/HA/Col composite scaffolds possess better cell adhesion and significantly higher proliferation and differentiation than the PHBV/HA composite scaffolds and the PHBV scaffolds. These results suggest that the PHBV/HA/Col composite scaffolds have a high potential for use in the field of bone regeneration and tissue engineering. Copyright © 2012 Jin-Young Baek et al.